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目的：建立测定战骨中柚皮素和芹菜素含量的高效液相色谱法。方法以 SHISEIDO￣SPOLAR C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相采用甲醇￣0.2％磷酸梯度洗脱(42：58),柚皮素的检测波长为288 nm,芹菜素的检测波长为340 nm,流速为1.0 mL.min－1,柱温为35℃,进样量10μL。结果柚皮素在0.180~3.60μg 之间线性关系良好,r＝0.9999,芹菜素在0.0052~0.1040μg 之间线性关系良好,r＝0.9999。结论该方法准确、可靠,可用于战骨药材及相关制剂中柚皮素和芹菜素的含量测定。“,”Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO￣SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol￣0.2% phosphoric acid (42:58) with isocratic elution at a flow rate of 1.0 mL.min-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection volumn was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 ~ 3.60 μg (r =0.999 9) and 0. 0052 ~ 0. 1040 μg ( r = 0. 999 9), respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.