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目的应用~(13)C核磁共振(13C-NMR)光谱测定鱼肝油中ω-3脂肪酸[十八碳四烯酸(C18∶4 n-3,moroctic acid,MA)、二十碳五烯酸(C20∶5 n-3,eicosapentaenoic acid,EPA)、二十二碳六烯酸(C22∶6 n-3,docosahexaenoic acid,DHA)]形成的甘油三脂中α(1,3)-酰基、β(2)-酰基的位次分布。方法用CDCl3溶解样品,利用高分辨核磁共振光谱直接测定。结果鱼肝油中MA、EPA和DHAα(1,3)-酰基、β(2)-酰基在δ171.5~173.5 ppm的化学位移与文献报道基本一致。2批次鱼肝油未发现上述ω-3脂肪酸特征峰,4批次鱼肝油有特征峰检出,但是β(2)-酰基的位次分布不同。按照β(2)-酰基的比例,4批鱼肝油来源于家养鱼类提取的可能性较大。结论应用该13C核磁共振技术可以鉴别鱼肝油的优劣,方法简单、快捷。
Objective To determine the content of ω-3 fatty acid in Cod liver oil (C18: 4 n-3, moroctic acid, MA), eicosapentaenoic acid (1,3) -acyl, β (beta) -acylglycerol in the triglyceride formed from C20: 5 n-3, eicosapentaenoic acid, EPA) and docosahexaenoic acid (DHA) (2) - Ordering of acyl groups. Methods The sample was dissolved in CDCl 3 and directly determined by high-resolution nuclear magnetic resonance spectroscopy. Results The chemical shifts of MA, EPA and DHAα (1,3) - acyl and β (2) - acyl in δ171.5 ~ 173.5 ppm were basically consistent with those reported in the literature. No significant peak of ω-3 fatty acid was found in 2 batches of cod-liver oil, and 4 batches of cod liver oil were detected with characteristic peaks, but the order distribution of β (2) -acyl was different. According to the ratio of β (2) -acyl groups, four batches of cod liver oil are more likely to be derived from domestic fish. Conclusion The 13C nuclear magnetic resonance technique can identify the advantages and disadvantages of cod liver oil, the method is simple and fast.