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【目的】探讨牛II型胶原诱导SD大鼠构建类风湿关节炎(Rheumatoid arthritis,RA)模型的可行性,为今后深入研究RA提供理想的动物模型。【方法】选择60只4周龄的SD大鼠,依据体重相近的原则随机分组(模型组50只,对照组10只),模型组通过注射牛Ⅱ型胶原建模,并通过体征观察、病理组织切片评价、钼靶影像学检测等判定建模是否成功。【结果】通过尾静脉注射牛Ⅱ型胶原免疫SD大鼠的建模成功率高达90.0%。与对照组SD大鼠相比,模型组SD大鼠体重减轻,活动减少,精神萎靡,先是后足小趾或踝关节红肿,之后炎症漫延至前肢,最终发展成慢性、多发性、对称性关节炎;模型组SD大鼠关节滑膜层次增多,且有大量炎性细胞浸润,软骨及骨组织受损,关节腔结构紊乱;模型组SD组大鼠从初次免疫第10周开始,其关节间隙狭窄、模糊,骨质疏松,关节软骨及骨组织受损。【结论】SD大鼠是研究RA发病机制及研发抗风湿药物的理想动物模型,可丰富该疾病动物模型的选择。
【Objective】 To investigate the feasibility of establishing a rheumatoid arthritis (RA) model induced by bovine type II collagen in SD rats and to provide an ideal animal model for in-depth study of RA. 【Methods】 Sixty four-week-old SD rats were selected and randomly divided into groups according to the similar body weight (50 in the model group and 10 in the control group). The model group was injected with bovine type Ⅱ collagen, Tissue section evaluation, molybdenum target imaging test to determine whether the model was successful. 【Results】 The success rate of modeling SD rats immunized with bovine type Ⅱ collagen by tail vein was as high as 90.0%. Compared with the control group, the SD rats in the model group lost weight, reduced activity and became asymptomatic. At first they were inflamed with the little toe or ankle of the hind paw. The inflammation then spread to the forelimbs, eventually developing into chronic, multiple and symmetrical joints The synovial layer of SD rats increased in model group with a large number of inflammatory cell infiltration, cartilage and bone tissue damage, and the structure of joint cavity was disorganized. SD rats in model group started from the tenth week after the first immunization, Stenosis, blurred, osteoporosis, articular cartilage and bone tissue damage. 【Conclusion】 SD rats are ideal animal models for studying the pathogenesis of RA and developing anti-rheumatoid drugs, which can enrich the animal model of disease.