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目的研究下咽鳞状细胞癌组织中神经迁移蛋白Slit2的表达及其与微血管密度的关系,探讨两者对下咽鳞状细胞癌的生物学行为的影响。方法免疫组织化学法检测Slit2蛋白的表达及微血管密度(MVD),并应用RT-PCR法检测新鲜组织中Slit2 mRNA的表达。研究其表达与肿瘤临床特征的关系。结果Slit2蛋白在下咽鳞状细胞癌和癌旁组织中的表达率分别为65.2%和40.5%,两者表达差异有统计学意义(χ2=6.329,P<0.05);Slit2 mR-NA在下咽鳞状细胞癌和癌旁组织中的表达,差异亦具有统计学意义(t=8.230,P<0.05)。Slit2蛋白的表达与肿瘤大小、TNM分期、淋巴结转移显著相关;MVD计数与肿瘤大小、浸润深度、TNM分期和淋巴节转移显著相关,两者与肿瘤的发病年龄、部位、临床病理分化程度均无关。肿瘤组织中Slit2阳性表达者MVD计数高于Slit2阴性表达者,其差异具有统计学意义(t=2.273,P<0.05);并且两者表达呈正相关(rs=0.430,P<0.05)。结论下咽鳞状细胞癌组织中Slit2表达和MVD计数,与肿瘤的侵袭和淋巴结转移密切相关,其机制可能为Slit-Robo信号系统介导了肿瘤新生血管的形成,从而影响了肿瘤的生物学行为。
Objective To investigate the expression of Slit2 in the squamous cell carcinoma of the hypopharynx and its relationship with the microvessel density (MVD), and to explore the influence of the two on the biological behavior of squamous cell carcinoma of the swallow. Methods Slit2 protein expression and microvessel density (MVD) were detected by immunohistochemistry. The expression of Slit2 mRNA in fresh tissues was detected by RT-PCR. To study the relationship between its expression and clinical features of tumors. Results The expression rates of Slit2 protein in squamous cell carcinoma and paracancerous tissue were 65.2% and 40.5%, respectively (χ2 = 6.329, P <0.05) The difference between the two groups was statistically significant (t = 8.230, P <0.05). Slit2 protein expression was significantly correlated with tumor size, TNM stage and lymph node metastasis. MVD count was significantly correlated with tumor size, depth of invasion, TNM stage and lymph node metastasis, both of which were not associated with the age, location of the tumor and clinical pathological differentiation . The MVD in Slit2 positive tumor was higher than that in Slit2 negative tumor (t = 2.273, P <0.05), and the positive correlation was found between them (rs = 0.430, P <0.05). Conclusions The expression of Slit2 and MVD in squamous cell carcinoma of the hypopharynx are closely related to tumor invasion and lymph node metastasis. The mechanism may be that the Slit-Robo signaling system mediates the formation of tumor neovascularization, which affects the biology of the tumor behavior.