We perform an experimental study of the multi-orbital effect on the high-order harmonic generation (HHG) from aligned N2 molecules in both linearly and elliptically polarized intense laser fields.Measured by a home-built extreme ultraviolet (XUV) flat grating spectrometer with the pump-probe method,the angular distributions of different orders of HHG are obtained,which show distinctive behaviors for harmonics in the plateau and the cut-off regions.The ellipticity dependence of HHG is investigated by aligning the molecular axis parallel or perpendicular to the laser polarization.Our results indicate that both the highest occupied molecular orbital (HOMO) as well as the lower one (HOMO-1) contribute to the HHG of N2 molecules,in either linearly or elliptically polarized intense laser field.The study paves the way for understanding the ultrafast electron dynamics of molecules exposed to an intense laser field.