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在传统的中药材对抗储藏法中,常用丹皮与泽泻对抗来防止泽泻生虫。在中药材仓库内丹皮与泽泻对抗储藏6个月后具有明显的防虫防蛀效果。为探明丹皮在对抗储藏系统中的防虫机制,本文采用McDonld(1970)的滤纸药膜法和Mondal(1984)的食物选择法测定丹皮提取物对几种中药材仓储害虫的忌避活性,结果发现药材甲、玉米象、谷蠹、赤拟谷盗、锯谷盗等5种仓虫的成虫对丹皮提取物3个浓度(100、200、400μg/cm2)处理的忌避率平均值均达V(80.1%~100%)级,进一步分析表明药材甲成虫的忌避率与丹皮提取物处理后周数呈极显著的负相关关系,赤拟谷盗幼虫对丹皮提取物3个浓度(0.25%、1.25%、2.5%)处理的忌避率平均值,一龄至六龄分别为Ⅱ(20.1%~40%)、Ⅲ(40.1%~60%)、Ⅲ、Ⅲ、Ⅳ(60.1%~80%)、Ⅴ级。同时还证明用试虫50%忌避率所需药物浓度(RC50)来比较不同药物对不同虫种的忌避活性高低是可行的,所得结论与方差分析结果完全一致。
In the traditional Chinese medicine confrontation storage method, commonly used Dan and Alisma confrontation to prevent Alisma parasite. In the Chinese herbal medicine warehouse Danza and Alisma confrontation storage 6 months after the obvious pest control moth effect. In order to find out the pest control mechanism in the storage system, we use the paper filter membrane method of McDonld (1970) and the food selection method of Mondal (1984) to determine the repellent activity of paeonol extract against several kinds of storage pests in Chinese herbal medicines. The results showed that the average of the repellent rates of three concentrations (100, 200 and 400 μg / cm2) of the adults of Radix Paeoniae Rubra, Up to V (80.1% ~ 100%). Further analysis showed that there was a significant negative correlation between the rejection rate of adult worm and the number of weeks after treatment. The average of the repellent rates of the first to the sixth instar of treatment were (Ⅱ) (20.1% ~ 40%), Ⅲ (40.1% ~ 60%), Ⅲ, Ⅲ, Ⅳ (60.1% ~ 80%), Ⅴ grade. At the same time, it was also proved that it is feasible to compare the repellent activity of different drugs against different insects by using the concentration of drug required to repel 50% of the test insects (RC50). The conclusion is consistent with the result of variance analysis.