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目的评价舒芬太尼后处理对缺血再灌注大鼠肌钙蛋白Ⅰ的影响,从而探讨其保护作用。方法健康雄性SD大鼠72只随机分为6组:假手术组(Sham组),缺血再灌注组(I/R组),缺血后处理组(Post组),舒芬太尼后处理组(LS组,MS组,HS组)。于缺血前即刻(T0,基础值)、缺血30 min(T1)、再灌注30 min(T2)、60 min(T3)、90 min(T4)和120 min(T5)时记录心率(HR)和平均动脉压(MAP);再灌注120 min末,随机取6只按照ELISA法测定心肌肌钙蛋白Ⅰ(cTnI)并计算心肌梗死面积(IS/AAR),另外6只进行光镜及电镜细胞形态学观察。结果 HR在各组各个时点比较差异无统计学意义(P>0.05),与Sham组比较,I/R组在T3、T4和T5时MAP均降低(P<0.05),而LS组在T3和T4时均降低(P<0.05);与I/R组相比,Post组、MS组和HS组血清中的cTnI、心肌梗死面积及心肌细胞损伤程度显著降低(P<0.05)。结论舒芬太尼后处理能够减轻在体大鼠心肌缺血再灌注损伤血清中cTnI,从而产生保护作用。
Objective To evaluate the effect of sufentanil postconditioning on the expression of troponin Ⅰ in rats with ischemia-reperfusion injury and to explore its protective effect. Methods Seventy-two healthy male Sprague-Dawley rats were randomly divided into 6 groups: Sham group, I / R group, Post-ischemic group, Post-sufentanil treatment Group (LS group, MS group, HS group). Heart rate (HR) was recorded immediately before ischemia (T0, basal), 30 min (T1), 30 min (T2), 60 min (T3), 90 min (T4) and 120 min (T5) ) And mean arterial pressure (MAP). At the end of 120 min after reperfusion, cardiac troponin Ⅰ (cTnI) was measured and the area of myocardial infarction (IS / AAR) was calculated according to ELISA. Observation of cell morphology. Results Compared with Sham group, HR of the I / R group was lower at T3, T4 and T5 (P <0.05), while there was no significant difference between HR group and control group (P <0.05). Compared with I / R group, the levels of cTnI, myocardial infarct area and cardiomyocytes in Post, MS and HS groups were significantly decreased (P <0.05). Conclusion Sufentanil postconditioning can reduce cTnI in the serum of myocardial ischemia-reperfusion injury in vivo, which has a protective effect.