Molecular Cloning of Human Sperm Surface Protein P34H Gene and Semi-quantitative Analysis of Its Exp

来源 :Asian Journal of Andrology | 被引量 : 0次 | 上传用户:yourice
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Objective: To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis. Methods: Total RNA was prepared from human epididymal tissues. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers and was then cloned into pGEM-T vector. The inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semiquantitative analysis of P34H expression in testis and caput, corpus and cauda epididymidis was done by RT-PCR based on bactin as an inner control. Results: Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank (Accession No. AF515625). Conclusion: The cloning of P34H gene may be useful for further basic and clinical studies on P34H Objective: To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis. Methods: Total RNA was prepared from human epididymal tissues. cDNA fragment encoding human P34H was amplified by RT -PCR using specific primers and was then cloned into pGEM-T vector. Also, semiquantitative analysis of P34H expression in testis and caput, corpus and cauda epididymidis was done by RT-PCR based on bactin as an inner control. Results: Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank (Accession No. AF515625). Conclusion: The cloning of P34H gene may be useful for further basic and clinical studies on P34H
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