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目的探讨抗端粒酶在肝细胞癌治疗中的意义。方法将反义hTR真核表达载体经脂质体介导转染人肝癌细胞系HepG2,体外培养及接种裸鼠观察其基因转染细胞的细胞周期、超微结构变化及致瘤性。结果形态学观察,转染后HepG2细胞出现典型的凋亡现象。FCM检测发现G1期前出现凋亡峰,凋亡率为4·2%。在裸鼠皮下的致瘤性明显降低。HepG2/pBBS212细胞的瘤体抑制率为2·4%,与HepG2/pBBS212-hTR细胞的25·6%相比,差异有显著性(P<0·05)。结论转染端粒酶反义RNA能抑制肝癌HepG2细胞的恶性表型,促进其凋亡。
Objective To investigate the significance of telomerase in the treatment of hepatocellular carcinoma. Methods Antisense hTR eukaryotic expression vector was transfected into human hepatocellular carcinoma cell line HepG2 by liposome. The cell cycle, ultrastructure and tumorigenicity of transfected cells were observed in vitro. Results Morphological observation showed that HepG2 cells showed typical apoptosis after transfection. The apoptosis peak appeared before G1 phase by FCM, and the apoptosis rate was 4.2%. Subcutaneous tumorigenicity in nude mice was significantly reduced. The inhibition rate of HepG2 / pBBS212 cells was 2.4%, which was significantly different from that of HepG2 / pBBS212-hTR cells (25.6%) (P <0.05). Conclusion Transfection of telomerase antisense RNA can inhibit the malignant phenotype of hepatocellular carcinoma HepG2 cells and promote its apoptosis.