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本文首次报道自制硷性磷酸酶(AKP)应用于ELISA间接法,将自制AKP与Siama(RZ=3)辣根过氧化物酶(HRP)分别用戊二醛一步法结合羊抗人IgG,两种酶结合物的最高滴度均达1:6,400,以蛋白含量4μg/ml的日本血吸虫卵粗抗原为包被抗原,每井0.2ml,用自制AKP和HRP结合物检测确诊血吸
This paper reports for the first time that the homemade alkaline phosphatase (AKP) was applied to ELISA indirect method. The homemade horseradish peroxidase (HRP) from AKP and Siama (RZ = 3) The highest titer of the enzyme conjugates reached 1: 6,400, with a protein content of 4μg / ml of Schistosoma japonicum egg antigen as coated antigen, 0.2ml per well, with homemade AKP and HRP conjugates confirmed blood tests