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在常规培养瓶中附贴小块盖玻片,使CNE细胞株在玻片上生长。培养6、24小时和2、3、4天后,各取出附在玻片的瘤细胞进行扫描电镜观察。检查结果,泡状突起和微绒毛是瘤细胞表面结构变化的主要特征。培养后一天,瘤细胞表面出现大量分布均匀的泡状突起,第二天开始分布于细胞周边,第三天后则几乎完全消失。而在培养第一天时,细胞边缘仅有少量微绒毛存在;到第二天时,微绒毛在细胞表面呈稀疏排列;第三天后,可见瘤细胞表面布满致密的微绒毛。此外,培养一天时,在细胞表面还查见叶状伪足和折叠皱纹状结构,后者于第二天消失。丝状伪足在不同培养时间内均可查见,但多分布于细胞一侧,也有的沿细胞周边分布。丝状伪足可见有2级分叉,其末端呈吸盘样结构。本实验表明,CNE细胞株的表面结构不是恒定不变的,而是具有明显的易变性,并可因培养时间的长短,而发生不同的改变。
Small coverslips were attached to conventional culture flasks to allow CNE cell lines to grow on glass slides. After culturing for 6, 24 and 2, 3 and 4 days, the tumor cells attached to the slide were removed for scanning electron microscopy. Check results, blisters and microvilli are the main features of the surface structure of tumor cells. One day after the culture, a large number of bubble-shaped protrusions appeared on the surface of the tumor cells, spreading around the periphery of the cells the next day, and disappeared almost completely after the third day. On the first day of culture, only a few microvilli existed at the edge of the cells. On the second day, the microvilli were sparsely arranged on the cell surface. On the third day, the surface of the tumor cells were covered with dense microvilli. In addition, on the day of culturing, leafy pseudopodia and folded wrinkle-like structures were observed on the cell surface, and the latter disappeared the next day. Fibrous filopodia in different culture time can be found, but mostly distributed in the cell side, and some along the cell periphery distribution. There are two levels of bifurcation of filopodia, and a sucker-like structure at the end. This experiment shows that the surface structure of CNE cell lines is not constant, but has obvious variability, and may vary due to the length of incubation time.