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目的:建立HPLC法测定五灵止痛胶囊中异鼠李素-3-O-新橙皮糖苷含量的方法。方法:色谱条件为:C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(14∶86),检测波长为254 nm,进样量10μl,流速1.0 ml/min,柱温30℃。结果:此方法线性范围为0.252~12.6μg(r=0.9998),平均回收率为99.05%,RSD=0.89%(n=6)。结论:所用方法测定分离效果好,重复性好,灵敏度高。
Objective: To establish a HPLC method for determination of isorhamnetin-3-O-neohesin in Wuling Zhitong Capsules. Methods: The chromatographic conditions were as follows: C18 column (150 mm×4.6 mm, 5 μm), the mobile phase was acetonitrile-water (14:86), the detection wavelength was 254 nm, the injection volume was 10 μl, the flow rate was 1.0 ml/min, and the column temperature was 30. °C. Results: The linear range of this method was 0.252~12.6μg (r=0.9998), the average recovery was 99.05%, and RSD was 0.89% (n=6). Conclusion: The method used to determine the separation effect is good, repeatability is good, and the sensitivity is high.