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目的探讨肺癌中支架蛋白IQGAP1的蛋白表达水平,及IQGAP1对肺癌细胞运动侵袭能力的影响。方法 RT-PCR检测IQGAP1mRNA的表达,免疫组织化学SABC法及Western blot检测IQ-GAP1蛋白的表达,并采用Transwell实验探讨IQGAP1蛋白促进侵袭转移的作用机制。结果研究发现其在肺癌组织存在高表达,IQGAP1蛋白可以通过提高肺癌细胞的运动能力促进肺癌的侵袭转移。结论 IQGAP1与肺癌密切相关,可能在肺癌的侵袭和转移中起重要做用。
Objective To investigate the protein expression of scaffold protein IQGAP1 in lung cancer and the effect of IQGAP1 on lung cancer cell motility and invasion. Methods The expression of IQGAP1 mRNA was detected by RT-PCR. The expression of IQ-GAP1 protein was detected by immunohistochemical SABC method and Western blot. The mechanism of IQGAP1 protein in invasion and metastasis was also explored by Transwell assay. Results The study found that it was highly expressed in lung cancer tissues. IQGAP1 protein could promote the invasion and metastasis of lung cancer by increasing the ability of lung cancer cells to exercise. Conclusion IQGAP1 is closely related to lung cancer and may play an important role in the invasion and metastasis of lung cancer.