论文部分内容阅读
目的 探讨 2型糖尿病患者脂蛋白酯酶 (LpL)、血管紧张素转换酶 (ACE)及载脂蛋白 E(ApoE)基因与冠心病的关系。方法 采用聚合酶链反应 限制性片段长度多态性 (PCR PFLP)、聚合酶链反应 (PCR)和聚合酶链反应 等位基因特异性寡核苷酸 (PCR ASO)探针杂交技术分别检测 2型糖尿病并冠心病组及对照组LpL、ACE、ApoE基因型。结果 ① 2型糖尿病并冠心病组LpL/P P 及P 频率分别为 18.2 %和 4 2 .9% ,高于对照组 (9.5 %和 32 .5 % ) ,但差异无统计学意义 (P >0 .0 5 )。LpL/HindⅢ多态性频率分布两组间无明显差异 (P >0 .0 5 )。② 2型糖尿病组ACE/DD及D频率分别为 33.8%和 5 7.1% ,明显高于对照组(12 .7%和 36 .5 % ) (P <0 .0 1)。③ 2型糖尿病并冠心病组ε 3/ 3和ε 4 / 3频率分别为 5 4 .8%和 31.2 % ,分别明显低于 (ε 3/ 3)和高于 (ε 4 / 3)对照组 (76 .19%和 11.11% ) (均为P <0 .0 1)。ε 4等位基因频率为 2 0 .8% ,明显高于对照组 (9.5 2 % ) (P =0 .0 1)。结论 LpL/ p 、ACE/D及ApoE/ε 4等位基因增高 2型糖尿病并发冠心病患者的遗传易感性。
Objective To investigate the relationship between lipoprotein lipase (LpL), angiotensin converting enzyme (ACE) and apolipoprotein E (ApoE) gene and coronary heart disease in type 2 diabetic patients. Methods Polymerase chain reaction restriction fragment length polymorphism (PCR-PFLP), polymerase chain reaction (PCR) and polymerase chain reaction allele-specific oligonucleotide (PCR ASO) Diabetes and coronary heart disease group and control group LpL, ACE, ApoE genotypes. Results ① The frequencies of LpL / PP and P in patients with type 2 diabetes mellitus and coronary heart disease were 18.2% and 42.9%, respectively, which were higher than those in the control group (9.5% and 32.5%), but the difference was not statistically significant (P> 0 .0 5). The frequency distribution of LpL / Hind Ⅲ polymorphism had no significant difference between the two groups (P> 0.05). ② The frequency of ACE / DD and D in type 2 diabetes mellitus were 33.8% and 51.1% respectively, significantly higher than those in control group (12.7% and 36.5%) (P <0.01). ③ The frequencies of ε 3/3 and ε 4/3 in type 2 diabetes mellitus and coronary heart disease were 54.8% and 31.2% respectively, which were significantly lower than those in (ε 3/3) and higher (ε 4/3) (76.19% and 11.11%) (all P <0.01). The ε 4 allele frequency was 20.8%, significantly higher than that of the control group (9.5 2%) (P = 0.01). Conclusion LpL / p, ACE / D and ApoE / ε 4 alleles increase the genetic predisposition of patients with type 2 diabetes mellitus complicated with coronary heart disease.