论文部分内容阅读
目的牛磺酸干预对亚慢性锰中毒大鼠基底前脑胆碱能神经细胞的影响。方法 SPF级雄性SD大鼠随机分为生理盐水对照组、染锰Ⅰ组、牛磺酸预防组、染锰Ⅱ组、牛磺酸治疗组。各组大鼠染毒结束后进行水迷宫实验检测大鼠空间学习记忆能力的变化,免疫组化染色观察胆碱乙酰转移酶(Choline acetyltransferase,ChaT)阳性神经元形态及数量变化。结果牛磺酸预防组平均逃避潜伏时间较染锰Ⅰ组显著缩短(P<0.05),牛磺酸治疗组与染锰Ⅱ组相比显著缩短(P<0.05),牛磺酸预防组平均平台搜索次数较染锰Ⅰ组增多(P<0.05),牛磺酸治疗组与染锰Ⅱ组相比差异无统计学意义(P>0.05)。染锰Ⅰ组基底节斜角带垂直支臂核(vDB)/水平支臂核(hDB)ChaT阳性细胞数明显少于对照组(P<0.05)。牛磺酸预防组hDB ChaT阳性细胞数较染锰组显著增多(P<0.05)。牛磺酸治疗组vDB ChaT阳性细胞数显著少于染锰Ⅱ组(P<0.05),而hDB ChaT阳性细胞数与染锰Ⅱ组无差异。结论牛磺酸预防或治疗改善染锰所致的学习记忆障碍,染锰致基底前脑ChaT阳性细胞显著减少,牛磺酸预防明显增加基底前脑hDB ChaT阳性细胞数量,而牛磺酸治疗组并未观察到胆碱能神经细胞增加。
Objective To investigate the effect of taurine on basal forebrain cholinergic neurons in rats with sub - chronic manganese poisoning. Methods SPF male SD rats were randomly divided into normal saline control group, manganese Ⅰ group, taurine prevention group, manganese Ⅱ group and taurine treatment group. Water maze test was used to detect the spatial learning and memory abilities of rats in each group. The morphology and number of ChT acetyltransferase (ChaT) positive neurons were observed by immunohistochemical staining. Results The average escape latency of taurine prevention group was significantly shorter than that of manganese Ⅰ group (P <0.05), taurine treatment group was significantly shorter than that of manganese Ⅱ group (P <0.05), taurine prevention group average platform The number of searches was more than that of Mn-Zn group (P <0.05), and there was no significant difference between taurine-treated and Mn-Mn-Zn groups (P> 0.05). The number of ChaT-positive cells in the VDDB / hDB group was significantly less than that in the control group (P <0.05). The number of hDB ChaT positive cells in taurine prevention group was significantly higher than that in manganese exposure group (P <0.05). The number of vDB ChaT positive cells in the taurine-treated group was significantly less than that in the manganese-exposed group Ⅱ (P <0.05), while the number of hDB ChaT positive cells was not different from that in the manganese-dyed Ⅱ group. Conclusion Taurine prevention or treatment can improve the learning and memory impairment caused by manganese, significantly reduce the number of ChaT-positive cells in the manganese-induced basal forebrain, and taurine prevention can significantly increase the number of hDB ChaT-positive cells in basal forebrain. However, No increase in cholinergic nerve cells was observed.