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本研究旨在建立一个适于分析大豆遭受大豆疫霉菌侵染后基因表达研究的互作体系。在比较了15个携带不同抗病基因的大豆基因型的组织培养情况和7个大豆疫霉菌分离物及其游动孢子单孢系的毒力变化的基础上,构建了大豆悬浮细胞和大豆疫霉菌游动孢子的共培养体系,进而分析了共培养过程中大豆细胞的活力和防御基因表达情况。结果表明,不同亲和力菌株的游动孢子引起的大豆细胞活力变化十分相似;非亲和菌株的游动孢子可诱导寄主防御基因的表达发生变化。此系统为深入开展大豆抗性机制研究提供了良好的平台。
The aim of this study was to establish an interaction system suitable for the analysis of gene expression in soybean infected with Phytophthora sojae. Based on the comparison of the tissue culture of 15 soybean genotypes with different disease resistance genes and the changes of virulence of seven Phytophthora sojae isolates and their zoospora sp. Strains, we constructed the soybean suspension cells and the soybean epidemic Mold zoospores co-culture system, and then analyzed the co-culture of soybean cells in vitality and defense gene expression. The results showed that the motility of soybean cells induced by zoospores of different avidity strains was very similar. The zoospores of non-affinity strains could induce the changes of host defense genes. This system provides a good platform for the further research on the mechanism of soybean resistance.