目的建立一个适于我国国情的血清氟分析标准方法。方法氟离子选择电极法。结果本方法的检测下限为０．０１２μｇ／ｍｌ。对氟含量为０．０５９、０．０９０μｇ／ｍｌ和０．２０３μｇ／ｍｌ的血清样品测定８次，相对标准偏差分别为３．３９％、１、８４％、０．９９％。对上述样品加标的回收率为９６．７％、１０１．２％、９９．８％；测定含氟１．０３μｇ／ｍｌ，５．０７μｇ／ｍｌ的牛血清游离氟成分标准物质相对偏差为４．１６％和１．０５％。本方法所规定的实验步骤及技术指标均经过实验室和现场验证。结论本方法是测定血清中游离氟的最简便、准确、灵敏度高的方法。 Objective To establish a standard method of serum fluorine analysis suitable for our national conditions. Methods Fluoride ion selective electrode method. Results The detection limit of this method was 0.012 μg / ml. The serum samples with Fluorine content of 0.059, 0.090μg / ml and 0.203μg / ml were measured 8 times, the relative standard deviations were 3.39%, 1,84% and 0.99% respectively. The spiked recoveries of the above samples were 96.7%, 101.2% and 99.8%, respectively. The relative standard deviation of free fluorine content of bovine serum samples with 1.03μg / ml fluoride and 5.07μg / ml was 4. 16% and 1.05%. The experimental procedures and technical indicators prescribed by this method have been verified by laboratory and field. Conclusion This method is the most simple, accurate and sensitive method for the determination of free fluorine in serum.