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目的建立大鼠脑组织中天麻苷元的HPLC测定方法,并研究川芎对天麻苷元在大鼠脑内药动学的影响。方法 144只SD大鼠随机分为天麻单用组(灌胃给予天麻提取物0.945 mg.kg-1)和天麻-川芎配伍组(灌胃给予天麻提取物0.945 mg.kg-1和川芎提取物3.78 mg.kg-1),于给药后不同时间点取脑组织。采用HPLC法测定大鼠脑组织中天麻苷元的浓度,以DAS2.0药动学软件计算药动学参数。结果天麻苷元在0.125～8 mg.L-1(r=0.999 6)范围内线性关系良好,绝对回收率为86.54%～89.96%,日内及日间精密度均低于5%。天麻-川芎配伍组中天麻苷元脑内药物浓度曲线下面积为天麻单用组的1.66倍;平均滞留时间延长,清除率为天麻单用组的64.58%,与天麻单用组相比有显著差异(均P<0.05)。结论川芎可提高天麻苷元在大鼠脑内的生物利用度,减缓天麻苷元在大鼠脑内的消除速度。
Objective To establish a HPLC method for the determination of genistein in rat brain and to study the effects of Chuanxiong on the pharmacokinetics of rat brain in vivo. Methods One hundred and fourty-four SD rats were randomly divided into Gastrodia alone (0.945 mg.kg-1) and Tianma-Chuanxiong compatibility group (intragastrically administered 0.945 mg.kg-1 and Rhizoma Chuanxiong Extract 3.78 mg.kg-1), brain tissue was taken at different time points after administration. The concentration of gastrodin in rat brain was determined by HPLC. The pharmacokinetic parameters were calculated by DAS2.0 pharmacokinetic software. Results The results showed that the linear range was 0.125 ~ 8 mg.L-1 (r = 0.999 6). The absolute recovery ranged from 86.54% to 89.96%. The intra- and inter-day precision was less than 5%. Gastrodia elata and Rhizoma Chuanxiong compatibility group, the space under the curve of drug concentration curve of the gastrodia agline group was 1.66 times; average retention time, clearance rate of 64.58% for gastrodia alone group, compared with gastrodin alone group Difference (all P <0.05). Conclusion Chuanxiong could increase the bioavailability of gastrodin in rat brain and slow the elimination of gastrodin in rat brain.