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目的鉴定河北中药材市场柴胡的真伪。方法对从河北中药材市场采购的28份柴胡药材的ITS2序列进行PCR扩增,同时从Gen Bank下载7个样本序列。采用Codon Code Aligner软件进行序列拼接,采用软件MEGA 6.0分析比对,计算其种间、种内的K2P距离以及各序列的变异位点并进行聚类分析。结果 28份样品中有21份为柴胡,3份为黑柴胡,4份为伪品。结论 ITS2序列能够作为柴胡药材鉴别的有效方法之一,对药材市场的规范和临床用药安全具有重要意义。
Objective To identify the authenticity of Bupleurum in Chinese herbal medicine market in Hebei province. Methods The ITS2 sequences of 28 Bupleurum medicinal materials purchased from Chinese herbal medicine market in Hebei were amplified by PCR, and 7 samples were downloaded from Gen Bank. The sequences were cloned by Codon Code Aligner software. The software MEGA 6.0 was used for analysis and comparison, and the distances between species and species within the species and the variation of each sequence were calculated and clustered. Results Among the 28 samples, 21 were Bupleurum, 3 were Black Bupleurum and 4 were counterfeit. Conclusion ITS2 sequence can be used as one of the effective methods to identify Bupleurum medicinal materials, which is of great significance to the standardization of medicinal materials market and the safety of clinical medication.