Background:Glehnia littoralis,as a traditional herbal medicine to heal various health ailments in East Asia,displays various therapeutic properties including antioxidant effects.However,neuroprotective effects of G.littoralis against cerebral ischemic insults have not yet been addressed.Therefore,in this study,we first examined its neuroprotective effects in the hippocampus using a gerbil model of transient global cerebral ischemia (TGCI).Methods:Gerbils were subjected to TGCI for 5 min.G.littoralis extract (GLE;100 and 200 mg/kg) was administrated orally once daily for 7 days before ischemic surgery.Neuroprotection was examined by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining.Gliosis was observed by immunohistochemistry for glial fibrillary acidic protein and ionized calcium-binding adapter molecule 1.For neuroprotective mechanisms,immunohistochemistry for superoxide dismutase (SOD) 1 and brain-derived neurotrophic factor (BDNF) was done.Results:Pretreatment with 200 mg/kg of GLE protected pyramidal neurons in the cu ammonis 1 (CA1) area from ischemic insult area (F=29.770,P ＜ 0.05) and significantly inhibited activationsof astrocytes (F =22.959,P ＜ 0.05) and microglia (F =44.135,P ＜ 0.05) in the ischemic CA1 area.In addition,pretreatment with GLE significantly increased expressions of SOD1 (F =28.561,P ＜ 0.05) and BDNF (F =55.298,P ＜ 0.05) in CA1 pyramidal neurons of the sham-and ischemia-operated groups.Conclusions:Our findings indicate that pretreatment with GLE can protect neurons from ischemic insults,and we suggest that its neuroprotective mechanism may be closely associated with increases of SOD 1 and BDNF expressions as well as attenuation ofglial activation.