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目的探讨趋化因子受体7(CCR7)和血管内皮生长因子-C(VEGF-C)在鼻咽癌组织中的表达及其与患者临床病理特征的关系。方法应用免疫组化En Vision两步法检测15例正常鼻咽黏膜组织及69例鼻咽癌组织中CCR7和VEGF-C的表达水平,分析其表达水平与鼻咽癌患者临床病理特征的关系。结果 CCR7及VEGF-C在鼻咽癌组织中呈高表达分别占39例(56.52%)、25例(36.23%),而在15例正常鼻咽黏膜组织中均呈低表达(P<0.05)。CCR7和VEGF-C表达与肿瘤淋巴结转移有关(P<0.05),与肿瘤临床分期、浸润深度、是否远处转移、患者年龄及性别等均无关(P>0.05);CCR7和VEGF-C蛋白表达呈显著相关性(r=0.366,P=0.002)。结论 CCR7和VEGF-C蛋白高表达与鼻咽癌淋巴结转移密切相关,可能促进淋巴管新生和癌细胞迁移等过程。
Objective To investigate the expression of chemokine receptor 7 (CCR7) and vascular endothelial growth factor-C (VEGF-C) in nasopharyngeal carcinoma and its relationship with clinicopathological features. Methods The expressions of CCR7 and VEGF-C in 15 cases of normal nasopharyngeal mucosa and 69 cases of nasopharyngeal carcinoma were detected by immunohistochemical En Vision two-step method. The relationship between the expression of CCR7 and VEGF-C and the clinicopathological features of NPC was analyzed. Results The high expression of CCR7 and VEGF-C in nasopharyngeal carcinoma were 39 (56.52%) and 25 (36.23%), respectively, which were all low in 15 cases of nasopharyngeal mucosa (P <0.05) . The expression of CCR7 and VEGF-C were correlated with tumor lymph node metastasis (P <0.05), and had no correlation with clinical stage, depth of invasion, distant metastasis, age and sex of the patients (P> 0.05) There was a significant correlation (r = 0.366, P = 0.002). Conclusion The high expressions of CCR7 and VEGF-C are closely related to lymph node metastasis of nasopharyngeal carcinoma, which may promote lymphangiogenesis and cancer cell migration.