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目的观察阿托伐他汀钙对载脂蛋白E基因缺陷(apoE-／-)小鼠动脉粥样硬化病变的抑制作用。方法将apoE-／-小鼠随机分为模型组和阿托伐他汀钙治疗组,每组各8只。酶法检测血清脂质含量,比色法测氧化指标一氧化氮,总抗氧化能力及丙二醛含量,病理图像分析法测定主动脉粥样硬化斑块面积及管腔面积,免疫组织化学染色方法测定主动脉壁血管细胞黏附分子-1、细胞间黏附分子-1的表达。结果阿托伐他汀钙高剂量组(5．0 mg·kg-1·d-1)、低剂量组(2．5 mg·kg-1·d-1)血浆一氧化氮含量分别为(27．3±9．1)mmol／L和(19．7±8．7)mmol／L,总抗氧化能力分别为(211．8±43．6)mmol／L和(177．4±39．6)mmol／L,比模型组[(3．5±2．4)mmol／L和(73．7±38．3)mmol／L]明显增高(P<0．01);丙二醛含量分别为(11．6±3．3)mmol／L和(14．4±3．4)mmol／L,比模型组(45．5±5．3)mmol／L明显降低(P<0．01)。阿托伐他汀钙可下调血管细胞黏附分子-1及细胞间黏附分子-1的表达(P<0．01)。结论阿托伐他汀钙能明显增强apoE-／-小鼠抗氧化能力,降低主动脉壁血管细胞黏附分子-1及细胞间黏附分子-1的表达,抑制动脉粥样硬化病变的发展。
Objective To investigate the inhibitory effect of atorvastatin calcium on the atherosclerotic lesions in apolipoprotein E gene deficient (apoE - / -) mice. Methods ApoE - / - mice were randomly divided into model group and atorvastatin calcium treatment group, with 8 mice in each group. Serum lipids were measured by enzyme-linked immunosorbent assay (ELISA), nitric oxide (NO), total antioxidant capacity and malondialdehyde (MDA) contents were measured by colorimetric method. Aortic atherosclerotic plaque area and luminal area were determined by histopathological analysis. Immunohistochemical staining Methods The expressions of cell adhesion molecule-1 and intercellular adhesion molecule-1 in the aortic wall were measured. Results The levels of plasma nitric oxide in the atorvastatin high-dose group (5.0 mg · kg-1 · d-1) and low dose group (2.5 mg · kg-1 · d-1) .3 ± 9.1 mmol / L and 19.7 ± 8.7 mmol / L, respectively. The total antioxidant capacity was (211.8 ± 43.6) mmol / L and (177.4 ± 39) mmol / 6) mmol / L, which was significantly higher than that of model group [(3.5 ± 2.4) mmol / L and (73.7 ± 38.3) mmol / L] (11.6 ± 3.3) mmol / L and (14.4 ± 3.4) mmol / L respectively, which was significantly lower than that of model group (45.5 ± 5.3) mmol / L (P < 01). Atorvastatin calcium down-regulated the expression of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 (P <0.01). Conclusions Atorvastatin calcium can significantly enhance the antioxidant capacity of apoE - / - mice and decrease the expression of vascular cell adhesion molecule - 1 and intercellular adhesion molecule - 1 in the aortic wall and inhibit the development of atherosclerotic lesions.