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目的:通过体外实验,探讨獐牙菜属植物抗HBV作用物质基础。方法:将贵州獐牙菜酮类提取物4、6分别作用于HBV DNA转染人肝癌细胞系2215细胞,通过检测第8 d的细胞培养液中HBsAg、HBeAg的变化来评价其抗HBV效果。结果:提取物4多数浓度对HBsAg、HBeAg表达有显著抑制作用(P<0.05~0.01),测得对HB-sAg、HBeAg的IC50分别为18.4μg/m l、22.8μg/m l;TI值分别大于6.8、5.5;提取物6多数浓度对HBsAg、HBeAg表达有极显著抑制作用(P<0.01),测得对HBsAg、HBeAg的IC50分别为16.1μg/m l、32.7μg/m l,TI值分别大于15.5、7.6。结论:酮类提取物4、6为体外抗HBV的活性成分。
Objective: To investigate the anti-HBV substance basis of Swertia species in vitro. METHODS: The anti-HBV effect was evaluated by detecting the changes of HBsAg and HBeAg in the cell culture medium on the 8th day after the 4th and 6th extractions of Guizhou wormkin flavonoids were treated with HBV DNA and transfected into human hepatoma cell line 2215 cells, respectively. . Results: Most concentrations of extract 4 significantly inhibited the expression of HBsAg and HBeAg (P<0.05~0.01). The IC50 of HB-sAg and HBeAg were 18.4 μg/ml and 22.8 μg/ml, respectively; 6.8, 5.5; most concentrations of extract 6 significantly inhibited the expression of HBsAg and HBeAg (P<0.01), and the IC50 values for HBsAg and HBeAg were 16.1 μg/ml and 32.7 μg/ml, respectively, and the TI values were greater than 15.5. 7.6. Conclusion: Anthraquinone extracts 4 and 6 are anti-HBV active ingredients in vitro.