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以云南龙陵铁皮石斛为试验材料,用溶剂浸提法提取铁皮石斛花色苷。通过单因素试验考察乙醇体积分数、料液比、提取温度、p H、提取时间对铁皮石斛花色苷提取率的影响,以正交试验进行工艺优化得到最佳提取工艺条件。并考察铁皮石斛花色苷对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑制作用。结果表明,溶剂浸提法提取铁皮石斛花色苷的最佳工艺条件为:60%乙醇、料液比为1 g:20 m L、提取温度40℃、p H 3、提取时间120 min、提取次数为2次,花色苷含量为3.12 mg/g FW。其对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的最低抑菌质量浓度分别为9.75 mg/L、4.88 mg/L和1.22 mg/L,最低杀菌质量浓度分别为9.75 mg/L、4.88 mg/L、2.44 mg/L。
Taking Dendrobium candidum from Longling, Yunnan as experimental materials, anthocyanins from Dendrobium officinale were extracted by solvent extraction. The effects of ethanol volume fraction, solid-liquid ratio, extraction temperature, p H and extraction time on the extraction rate of anthocyanin from Dendrobium officinale were investigated by single factor experiment. The optimal extraction conditions were obtained through orthogonal experiment. The inhibitory effects of anthocyanins from Dendrobium officinale on Escherichia coli, Staphylococcus aureus and Bacillus subtilis were also investigated. The results showed that the optimal extraction conditions of anthocyanin from Dendrobium officinale with solvent extraction were as follows: 60% ethanol, solid-liquid ratio 1 g: 20 m L, extraction temperature 40 ℃, p H 3, extraction time 120 min, 2 times, anthocyanin content was 3.12 mg / g FW. The minimum inhibitory concentration against Escherichia coli, Staphylococcus aureus and Bacillus subtilis were 9.75 mg / L, 4.88 mg / L and 1.22 mg / L respectively, and the minimum bactericidal concentrations were 9.75 mg / L and 4.88 mg / L, 2.44 mg / L.