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[目的]探讨外耳道乳头状瘤(PEAC)的病因机制,分析外耳道乳头状瘤与人乳头状瘤病毒(HPV)感染组织病理学基础.[方法]选取2011年1月至2016年12月本院收集的130例PEAC手术切除标本,采用 HE染色和原位杂交技术检测低危型病毒(HPV6/11)DNA 感染和高危型病毒(HPV16/18)DNA 感染情况.[结果]PEAC伴挖空细胞低危型 HPV6/11DNA感染率(79.52%)显著高于 PEAC 不伴挖空细胞(P 0.05);男性在 HPV6/11DNA感染率(80.21%)显著高于女性(47.06%)(P 0.05);HE染色结果显示,鳞状上皮呈乳头状增生,表皮轻度角化或重度角化,表层有角化不全细胞散在,中心结缔组织有淋巴细胞浸润,表皮浅层或中层细胞可见大量挖空细胞,真皮乳头部毛细血管增生扩张;原位杂交结果显示,HPV6/11阳性呈现细胞核阳性,而HPV16/18阳性主要呈现细胞质阳性.[结论]PEAC的致病机制可能由 HPV病毒感染所致.“,”[Objective]To investigate the etiology and pathogenesis of papilloma of external auditory canal (PEAC),and analyze the relationship between the PEAC and human papilloma virus (HPV)infection.[Methods]From January 2011 to December 2016,130 PEAC surgical specimens were collected from our hospital.The infec-tion of HPV6/11 DNA and HPV16/18 DNA infection were detected by HE staining and in situ hybridization.[Results]The low-risk HPV6/11 DNA infection rate of PEAC with koilocyte (79.52%)was significantly higher than that of PEAC without koilocyte (P 0.05).The HPV6/11DNA infection rate in men was significantly higher than that in women (80.21% vs 47.06%),but there was no statistically significant difference between men and women in the HPV16/18 DNA infection rate (21.87% vs 14.71%).HE staining showed that squamous epithelium was papillary hyper-plasia,mild or severe hyperkeratosis of epidermis,parakeratosis cells scattered in epithallium,lymphocytes infil-tration in central connective tissues,a large number of koilocytes in epidermal superficial or middle-level cells and dermal papilla hyperplasia and dilatation of capillaries.The results of in situ hybridization found that HPV6/1 1 positive cells showed positive nucleus,while HPV16/18 positive cells showed positive cytoplasm.[Conclusion]The etiology and pathogenesis of PEAC may due to HPV virus infection.