采用单粒传的方法从大白菜耐热品种 177和热敏感品种 2 76杂交后代获得遗传性稳定的重组近交系群体 ,以此为材料用同工酶以及RAPD和AFLP分子标记技术鉴定了与大白菜耐热性数量性状相关的遗传标记 ,单因子方差分析和多元线性回归分析的结果表明 ,有 9个与耐热性QTL紧密连锁的分子标记 ,包括 5个AFLP标记 ,3个RAPD标记和 1个PGM同工酶标记 ,这些标记对耐热性遗传的贡献率为 4 6 .7%。 9个标记中有 5个分布在同一连锁群上 ,其他4个标记与任何一个标记无连锁关系 ,表明上述 9个标记分布在大白菜的 5个连锁群上。 A single recombinant inbred line was obtained from the progeny of resistant varieties of Chinese cabbage (177) and heat-sensitive variety (2 of 76), and was used as material to identify isozymes with isozyme and RAPD and AFLP molecular markers The results of single factor ANOVA and multivariate linear regression analysis showed that there were 9 molecular markers closely linked to the heat tolerance QTLs, including 5 AFLP markers, 3 RAPD markers and 1 PGM isoenzyme marker. The contribution of these markers to heat tolerance was 46.7%. Five of the 9 markers were distributed on the same linkage group, and the other 4 markers were not linked with any of the markers, indicating that the above 9 markers were distributed on 5 linkage groups of Chinese cabbage.