论文部分内容阅读
目的建立潜水减压病(DCS)猪模型,用于应用基础研究。方法选用6头巴马小型猪,采用DWC150型动物实验舱模拟潜水方案处理,以压缩空气加压至600kPa,停留30 min后,以600~500kPa 1 min、500~400kPa 1.5 min、400~300kPa 2min、300~200kPa 2.5min、200~100kPa 3min的方案减压。用动物跑步机评测肢体运动功能,诱发电位仪检测脊髓诱发电位联合确定DCS脊髓损伤;超声评估右心气泡量和心肺功能;观察皮肤症状演变并检测血液指标。通过整体行为学、组织病理学、循环和神经生理学、气泡生成、炎症反应等方面筛选建立评估指标体系。结果模型DCS发病率100%,死亡率0%。运动功能和诱发电位在减压前后发生明显变化;超声检测出减压后静脉系统气泡量显著,肺动脉指标改变;同时出现典型皮肤损伤;白细胞、血小板数量减少,D-二聚体量增高,肺、脊髓发生淤血、出血等病理改变。上述各类指标能够客观反映模型损伤,可以组成衡量模型发病的评估体系。结论巴马猪是制备DCS大动物模型的理想动物。
Objective To establish a model of subcutaneous decompression sickness (DCS) pigs for the application of basic research. Methods Six Bama miniature pigs were selected and treated with the simulated diving program of DWC150 animal experiment chamber. After the compressed air was pressurized to 600kPa for 30 min, the animals were immersed in 600 ~ 500kPa for 1 min, 500 ~ 400kPa for 1.5 min and 400 ~ 300kPa for 2min , 300 ~ 200kPa 2.5min, 200 ~ 100kPa 3min decompression. The animal treadmill was used to evaluate the motor function of the limbs. The evoked potentials were used to detect spinal cord evoked potentials to determine the DCS spinal cord injury. The amount of right ventricular airway and cardiopulmonary function were evaluated by ultrasound. The evolution of skin symptoms and blood parameters were observed. Through the overall behavior, histopathology, circulation and neurophysiology, bubble formation, inflammation and other aspects of screening evaluation index system. Results The incidence rate of DCS was 100% and the mortality rate was 0%. Motor function and evoked potentials were significantly changed before and after decompression; ultrasonically detected venous system after decompression was significant volume of the pulmonary artery index changes; while typical skin lesions; white blood cells, platelet count decreased, increased D-dimer, lung , Spinal cord congestion, bleeding and other pathological changes. The above indicators can objectively reflect the damage of the model and can form an evaluation system to measure the incidence of the model. Conclusion Bama pig is an ideal animal for the preparation of DCS animal model.