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目的:建立HPLC法同时测定野菊花中绿原酸和蒙花苷的含量。方法:色谱柱:Agilent Eclipse XDB-C_(18)(250 mm×4.6 mm,5μm);流动相:甲醇-乙腈-0.05%磷酸溶液,梯度洗脱;检测波长:326 nm;流速:1.0 ml·min~(-1);进样量:10μl;柱温:35℃。结果:绿原酸和蒙花苷的线性范围分别为3.476×10~(-2)～0.695 2μg(r=1.000 0,n=7)和8.957×10~(-2)～1.792μg(r=1.000 0,n=7);提取回收率分别为98.79%(RSD=1.0%,n=6)和99.39%(RSD=0.5%,n=6)。结论:本法操作简便、快速、结果准确,可用于野菊花的质量控制。
Objective: To establish a HPLC method for the simultaneous determination of chlorogenic acid and mandarin in wild chrysanthemum. Method: Column: Agilent Eclipse XDB-C 18 (250 mm × 4.6 mm, 5 μm); mobile phase: methanol-acetonitrile-0.05% phosphoric acid solution with gradient elution; detection wavelength: 326 nm; min ~ (-1); injection volume: 10μl; column temperature: 35 ℃. RESULTS: The linear ranges of chlorogenic acid and monatin were 3.476 × 10 -2 ~ 0.695 2 μg (r = 1.000 0, n = 7) and 8.957 × 10 -2 ~ 1.792 μg (r = 1.000 0, n = 7). The recoveries were 98.79% (RSD = 1.0%, n = 6) and 99.39% (RSD = 0.5%, n = 6) respectively. Conclusion: This method is simple, rapid, accurate and can be used for the quality control of wild chrysanthemum.