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为了解青海省藏羊、牦牛和高原鼠兔棘球蚴的感染状况及藏羊和牦牛细粒棘球蚴的优势虫株,本研究对青海省祁连县、泽库县、贵南县和玉树县的藏羊或牦牛以及称多县的高原鼠兔棘球蚴感染状况进行了调查,并应用线粒体细胞色素氧化酶Ⅰ基因(CO1)和NADH脱氢酶亚单位Ⅰ基因(ND1)对11个棘球蚴分离株(牦牛7个分离株,藏羊3个分离株,高原鼠兔1个分离株)进行测序与鉴定并构建了系统发育树。结果显示,调查地区藏羊棘球蚴的平均感染率为52.30%,牦牛棘球蚴的平均感染率为36.77%,高原鼠兔棘球蚴平均感染率为4.14%;藏羊和牦牛源分离株为细粒棘球蚴普通羊株(G1型),CO1基因变异率为0.3%~0.6%、ND1基因变异率为0~0.4%,高原鼠兔源分离株为石渠棘球蚴,CO1基因变异率为0.5%、ND1基因变异率为1.4%。结果表明家畜棘球蚴感染率高,危害大,并且均为细粒棘球绦虫普通羊株(G1型);高原鼠兔棘球蚴感染率不高,但其在野生动物与野生动物及野生动物与家畜之间棘球蚴病的传播中占有重要的地位。
In order to understand the infection status of Echinococcus granulosus in Tibetan sheep, yaks and plateau in Qinghai Province and the dominant strains of Echinococcus granulosus in Tibetan sheep and Yak, this study studied the genetic diversity of Tibetan macaques in Qilian, Zeku, Guinan, Echinococcus granulosus infection in Tibetan sheep or yak in Yushu county and plateau in the counties were investigated. Mitochondrial cytochrome oxidase Ⅰ (CO1) gene and NADH dehydrogenase subunit Ⅰ gene (ND1) A total of 9 strains of yak, 7 yaks, 3 Tibetan sheep and 1 plateau pika were sequenced and identified and phylogenetic trees were constructed. The results showed that the average infection rate of Echinococcus granulosus was 52.30%, the average infection rate of Echinococcus granulosus was 36.77%, and the infection rate of Echinococcosis in plateau was 4.14%. The Tibetan sheep and yak source isolates (G1), the mutation rate of CO1 gene was 0.3% ~ 0.6%, the mutation rate of ND1 gene was 0 ~ 0.4%. The rabbit plateau pupal isolates were Echinococcus granulosus, CO1 gene The mutation rate was 0.5% and the mutation rate of ND1 gene was 1.4%. The results showed that the infection rate of hydatid cysts in domesticated animals was high and the harm was very high. All of them were normal sheep strains (G1 type) of Echinococcus granulosus. The infection rate of hydatid cysts in plateau was not high, but in wild animals and wild animals Animal and livestock among the echinococcosis plays an important role.