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目的观察叶酸(FA)、维生素B6(VB6)、维生素B12(VB12)对同型半胱氨酸(Hcy)上调内皮细胞血管细胞粘附分子-1(VCAM-1)表达抑制作用。方法将对数生长期大鼠主动脉内皮细胞随机分为5组,空白对照组、1mmol/LHcy组、抑制组Ⅰ(1mmol/LHcy+5μmol/LFA)、抑制组Ⅱ(1mmol/LHcy+5μmol/LFA+5nmol/LVB12)、抑制组Ⅲ(1mmol/LHcy+5μmol/LFA+0.1μmol/LVB6+5nmol/LVB12)、逆转录聚合酶链反应(RT-PCR)检测血管细胞粘附分子VCAM-1的表达。结果与空白对照组比较,1mmol/LHcy明显上调核转录因子κb(NF-κb)和VCAM-1mRNA的表达,内皮-单核细胞粘附率由6.03%增加到59.04%(P<0.05);与1mmol/LHcy组比较,3个抑制组NF-κb(F=11.547,P=0.000)、VCAM-1mRNA(F=94.014,P=0.000)的表达均明显下调,抑制组Ⅰ、Ⅱ、Ⅲ的内皮-单核细胞的粘附率分别为44.98%,27.23%,10.83%,均明显低于1mmol/LHcy组(P<0.05)。结论叶酸、VB6、VB12均可抑制NF-κb、VCAM-1 mRNA的表达,减少内皮-单核细胞的粘附,缓解Hcy对血管内皮细胞的损伤作用,且三者同时使用效果更好。
Objective To investigate the inhibitory effects of folic acid (FA), vitamin B6 (VB6) and vitamin B12 (VB12) on the upregulation of vascular cell adhesion molecule-1 (VCAM-1) expression in endothelial cells. Methods Aortic endothelial cells of rats in logarithmic growth phase were randomly divided into 5 groups: blank control group, 1 mmol / LHcy group, 1 mmol / L LHcy + 5 μmol / L FA group, LFA + 5nmol / LVB12), group Ⅲ (1mmol / L LHcy + 5μmol / LFA + 0.1μmol / LVB6 + 5nmol / LVB12) and the expression of vascular cell adhesion molecule VCAM-1 by reverse transcription polymerase chain reaction expression. Results Compared with the blank control group, 1mmol / LHcy significantly increased the expression of nuclear factor kappa B (NF-κB) and VCAM-1 mRNA, and the adhesion rate of endothelial cells increased from 6.03% to 59.04% (P <0.05) The expression of NF-κB (F = 11.547, P = 0.000), VCAM-1mRNA (F = 94.014, P = 0.000) The adhesion rate of monocytes was 44.98%, 27.23% and 10.83% respectively, which were significantly lower than that of 1mmol / LHcy group (P <0.05). Conclusion Folic acid, VB6 and VB12 can both inhibit the expression of NF-κB and VCAM-1 mRNA, reduce the adhesion of endothelial cells and monocytes, and relieve the injury of vascular endothelial cells induced by Hcy.