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黄萎病菌培养滤液对陆地棉珂字２０１的愈伤组织及悬浮细胞系的生长和生活力有显著的影响。低浓度的病菌培养滤液，可抑制愈伤组织和悬浮细胞的生长，生活力下降；高浓度则可使细胞死亡。珂字２０１愈伤组织经诱变，在含１０％～２０％的黄萎病菌培养滤液的培养基上悬浮培养，经多次筛选获５个抗性细胞系。经鉴定，Ｍ２１０１０１５、Ｍ１１０１０１０１０和Ｍ１２０１０三个细胞系在１０％病菌培养滤液培养基上的存活率，由珂字２０１的３４．８５％提高到８１．２５％、８５．００％和８０．００％，且高于耐病品种爱ＳＪ—１（６３．５２％）和ＳＪ—５（５６．６７％）；它们的原生质体对病菌培养滤液的敏感性降低。抗性细胞系植株再生研究表明，其胚胎发生能力均比对照下降，畸胚、畸苗增多。Ｎ１１０１０的再生植株，经鉴定，对黄萎病菌培养滤液的反应是发病时间推迟，萎蔫程度明显减轻，表现一定的抗性。
The filtrate of Verticillium dahliae had a significant effect on the growth and viability of callus and suspension cell line of cotton cultivar “Kedi 201”. Low concentrations of bacteria culture filtrate, can inhibit the growth of callus and suspension cells, decreased viability; high concentrations can make the cell death. Ke word 201 callus by mutagenesis, containing 10% to 20% of Verticillium wilt culture filtrate suspension culture, after repeated screening of five resistant cell lines. It was identified that the survival rates of M2101015, M110101010 and M12010 cell lines on the 10% bacteria culture filtrate medium increased from 34.85% to 87.25%, 85.00% and 80.00% (63.52%) and SJ-5 (56.67%), respectively. The protoplasts were less susceptible to the bacteria culture filtrate. Studies on plant regeneration of resistant cell lines showed that the embryogenic capacity of the resistant cell lines was lower than that of the control, and the number of abnormal embryos and seedlings was increased. N11010 regenerated plants, identified, on the reaction of Verticillium wilt culture filtrate is the onset time delayed wilting significantly reduced, showing some resistance.