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以唐菖蒲花色突变株开花后的子房为外植体,接种于MS+BA1.0(单位mg/L,下同)+NAA0.01—0.1或MS+BA1.0+KT1.0+NAA0.01—0.1培养基中,从膨大的组织表面直接诱导不定芽。每隔4周将膨大组织或具有不定芽的组织转入1/2MS+BA1.0+NAA0.01—0.1培养基中,不断地诱导出不定芽。分化的不定芽转入不含激素的1/2MS培养基中,形成幼苗,然后生根,形成小球茎。或将幼苗转入1/2MS+NAA0.1培养基中,形成壮苗,然后生根,最后形成小球茎。1个唐菖蒲突变株子房,经6—7个月培养,获得了上千株试管苗。
The ovary after flowering of the gladiolus mutant was used as explants and inoculated on MS + BA1.0 (mg / L, the same below) + NAA0.01-0.1 or MS + BA1.0 + KT1.0 + NAA0 Adipose shoots were induced directly from the enlarged tissue surface in culture medium .01-0.1. Adventitially induced adventitious buds were continually transplanted into tissues of 1 / 2MS + BA1.0 + NAA0.01-0.1 at 4 weeks intervals with expanded or adventitious buds. Differentiated adventitious shoots were transferred to hormone-free 1 / 2MS medium to form seedlings and then rooted to form small bulbs. Or the seedlings transferred to 1 / 2MS + NAA0.1 medium to form strong seedlings, and then rooting, the formation of small bulbs. A gladiolus mutant ovary, after 6-7 months of culture, obtained thousands of plantlets.