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目的探究耶尔森菌对腹泻粪便中小肠结肠炎耶尔森氏菌的诊断价值。方法随机选取2014年4月~2015年5月入诊我院治疗的582例腹泻患者进行反转录PCR(RT-PCR)以及培养方法进行小肠结肠炎耶尔森氏菌肠炎的相关检验,对比两种方式的检验检出率,并总结其临床特征。结果相比较采取培养法(检出率为6.01%),RT-PCR具有更高的检出率,为9.62%,差异较大,P<0.05。所有使用RT-PCR检测阳性的患者中,以儿童为主,患儿主要症状为胃肠炎、阑尾炎,并有29例肠外并发症的出现。结论对小肠结肠炎耶尔森菌检测时使用RT-PCR检出率更佳,可临床推广。
Objective To investigate the diagnostic value of Yersinia pestis against Yersinia enterocolitica in diarrhea stool. Methods 582 cases of diarrhea patients admitted to our hospital from April 2014 to May 2015 were randomly selected for reverse transcription PCR (RT-PCR) and culture methods for the detection of Yersinia enterocolitica of enterocolitis. Two ways to test the detection rate, and summarizes its clinical features. Results Compared with the culture method (detection rate of 6.01%), RT-PCR has a higher detection rate of 9.62%, a significant difference, P <0.05. Among all patients who tested positive by RT-PCR, children were predominant. The main symptoms in children were gastroenteritis and appendicitis, and there were 29 cases of extraintestinal complications. Conclusion The detection rate of Yersinia enterocolitica using RT-PCR is better and can be clinically promoted.