Role of stress-activated MAP kinase P38 in cisplatin-and DTT-induced apoptosis of the esophageal car

来源 :世界胃肠病学杂志(英文版) | 被引量 : 0次 | 上传用户:hhz20091
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
AIM: To study the role of P38 kinase in esophageal cancer cell apoptosis induced by genotoxin, cisplatin and the unfolded protein response (UPR) inducer, dithiothreitol(DTT).METHODS: Esophageal carcinoma cell line Eca109 was cultured in RPMI 1640 medium to 70% confluency and treated with either cisplatin, DTT, or cisplatin plus DTT in the presence or absence of P38 inhibitor, SB203580. The untreated cells served as the control. The esophageal carcinoma cell apoptosis was detected by agarose gel DNA ladder analysis and quantified by flow cytometry.The P38 phosphorylation was detected by immunohis-tochemistry using antibodies specific to phosphorylated P38 protein.RESULTS: (1) Both cisplatin and DTT induced apoptosis in the esophageal cancer cell line Eca109 as shown by DNA ladder formation; (2) As detected by antibodies specific for the phosphorylated P38 protein (p-P38), both cisplatin and DTT treatments activated the stress-activated enzyme, MAP kinase P38. The number of positive cells was about 50% for the treatment groups, comparing to that of 10% for untreated group. DTT treatment, but not cisplatin treatment, induces nuclear localization of p-P38;(3) As measured by flow cytometry, inhibition of P38 activity by SB203580 blocks DFT- and cisplatin-induced apoptosis.The rates for DTT, cisplatin, and DTT plus cisplatin-induced apoptosis were 16.8%, 17.1%, and 21.4%, respectively.Addition of the SB compound during the incubation reduced the apoptotic rate to about 7.6% for all the treatment groups, suggesting that P38 activation is essential for cisplatin- and DTT-induced apoptosis in Eca109 cells.CONCLUSION: (1) Both DTT and cisplatin were able to induce apoptosis in esophageal cancer cell line Eca109;(2) P38 MAP kinase is essential for DTT- and cisplatin-induced apoptosis in Eca109 cells; (3) P38 activation may be the common signaling component relaying the multiple upstream signaling events to the downstream cell death program.
其他文献
目的 讨论DR系统胸部摄影在肺结核病筛查中的应用价值.方法 现随机选取90例于2016年6月-2017年7月期间在我院接受肺结核病筛查的患者作为本次调查研究样本,根据患者的检查时
笔者的观点是:医生集团走上“网红”之路,但对医改的意义却很有限.rn在医生集团里,以专科医生、高职能的医生为主,比如张强医生,他本身就是我国血管外科领域的知名专家.他们
期刊
期刊
患者男,55岁.主因“右枕颞部疼痛8个月伴双下肢无力3个月余”入院.患者于入院前8个月无明显诱因突发右枕颞部疼痛,性质为钝痛,休息后可缓解,之后间断性发作数次,程度逐渐加重,部位性质同前,缓解时间逐渐延长,偶尔疼痛时眩晕、倒地,意识不清,牙关紧闭,双眼上翻,恶心呕吐,呕吐物为胃内容物,甘露醇治疗缓解.1个月前自觉双下肢无力,左侧为著,记忆力下降.入院查体:各项生命体征未见明显异常,神清语利,精神好
期刊
目的:探究人性化服务在精神护理管理中的应用价值.方法:本次选取精神病患者86例作为研究对象,分为两组,即观察组给予人性化服务、对照组给予常规护理措施;对比两组患者结果.
目的:探究优质护理在外伤性急性硬膜外血肿护理中的应用.方法本次研究对象为我院在2017年9月至2018年9月治疗的70例外伤性急性硬膜外血肿患者,将其根据不同的护理方案平均分
目的 探讨非药物康复疗法在2型糖尿病患者中的影响.方法 选取我院收治的2型糖尿病患者110例,随机分为两组,对照组50例,行常规药物治疗,观察组50例,在对照组的基础上行非药物
目的:探讨跟骨骨折畸形愈合的数字化虚拟重建的技术方法及其在矫形手术设计中应用的可行性。方法利用跟骨骨折畸形愈合病例患侧、健侧足踝部CT扫描图像及其数据收集,应用Mimics