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选取银杏叶片为试验材料,研究纤维素酶Umcel9y-1在银杏叶片总黄酮提取中的应用价值。试验结果表明,0.08 mg·mL~(-1)纤维素酶Umcel9y-1,在其最适反应条件下(pH7.0,37oC),处理银杏叶片1 h、2 h、4 h和过夜(12 h),总黄酮的提取量较对照组分别提高了20.3%、20.7%、16.8%和15.0%。纤维素酶试验组处理2 h,总黄酮的提取量可以达到对照组12 h提取所得总黄酮量的97.0%,纤维素酶Umcel9y-1显著缩短了银杏叶总黄酮提取时间。采用超高效液相色谱对2种提取方法获得的提取液进行药用成分检测,发现添加纤维素酶Umcel9y-1对中药提取液活性成分没有显著的影响。
Ginkgo biloba leaves were selected as experimental materials to study the application value of cellulase Umcel9y-1 in the extraction of total flavonoids from leaves of Ginkgo biloba. The results showed that under the optimal reaction conditions (pH7.0, 37oC), the enzyme activity of 0.08 mg · mL -1 Umcel9y-1 was 1 h, 2 h, 4 h and 12 h h), the extraction of total flavonoids than the control group increased by 20.3%, 20.7%, 16.8% and 15.0%. Cellulase enzyme treatment group 2 h, the extraction of total flavonoids can reach the control group 12 h extraction of the total flavonoid content of 97.0%, cellulase Umcel9y-1 significantly shortens the ginkgo leaf flavonoids extraction time. The ultra-high performance liquid chromatography (HPLC) was used to detect the medicinal components of the extracts obtained from the two extraction methods. It was found that the addition of cellulase Umcel9y-1 had no significant effect on the active ingredients of the Chinese herbal extracts.