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对防治水产动物细菌性败血病的中草药复方总黄酮的提取条件的进行了优化研究。正交试验的结果表明:用30倍量的85%的乙醇在76℃条件下回流提取3次,每次2h为最佳提取条件。
The optimal conditions for the extraction of total flavonoids from Chinese herbal medicine against bacterial septicemia in aquatic animals were optimized. Orthogonal test results show that: 30 times the amount of 85% ethanol at 76 ℃ refluxed three times, each 2h for the best extraction conditions.