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目的 观察 ＨＢＶ ＤＮＡ 疫苗（ｐＣＲ３－１Ｓ） 诱导Ｂａｌｂ／ ｃ 小鼠（ Ｈ２ｄ） 的特异性细胞免疫应答及其对稳定表达ＨＢｓＡｇ 的小鼠肥大细胞瘤Ｐ８１５ 细胞（Ｐ８１５ＨＢＶＳ） （ Ｈ２ｄ） 成瘤性的影响．方法 肌肉注射ＤＮＡ 疫苗，背部皮下接种Ｐ８１５ＨＢＶＳ 细胞，观察成瘤情况，４ ｈ ５１Ｃｒ 释放法检测小鼠脾细胞ＣＴＬ 活性．结果 接种 ＤＮＡ 疫苗后小鼠成瘤率为１２－５ ％ ， 对照组为１００ ％ ． 小鼠平均存活期大于３８－２ ｄ ，对照组为２８－４ ｄ ，４０ ｄ 后小鼠存活率为８７－５ ％ ，对照组为０ ％ ． ＣＴＬ 细胞杀伤活性明显增加，ｐＣＲ３－１Ｓ 组５１ ％ ，对照组为２１ ％ （ Ｐ＜ ０－００１） ．结论 ＤＮＡ 疫苗可以诱导细胞免疫应答，对体内ＨＢＶ 感染具有预防及治疗作用．
Objective To observe the specific cellular immune response induced by HBV DNA vaccine (pCR3-1S) in Balb / c mice (H2d) and its effect on the expression of HBsAg in mouse mastocytoma P815 cells (P815HBVS) (H 2d) tumorigenicity. Methods The DNA vaccine was injected intramuscularly and the P815HBVS cells were subcutaneously inoculated into the back of the mice. The tumorigenesis was observed. The CTL activity of splenocytes was detected by 51Cr release assay. Results The rate of tumorigenesis was 12-5% in mice vaccinated with DNA vaccine and 100% in control group. The average survival time was longer than 38-2 days in mice and 28-4 days in control group. After 40 days, the survival rate was 87-5% in mice and 0% in control group. CTL cell killing activity was significantly increased in 51% of pCR3-1 S group, the control group was 21% (P <0-001). Conclusion DNA vaccine can induce cellular immune response and has the preventive and therapeutic effects on HBV infection in vivo.