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本实验以大白菜“０２号杂交早皇白”无菌苗的子叶作为材料，就组织培养过程中影响芽再分化的各种因素进行了初步探讨．用附加ＢＡ４．４×１０－６～３５．５×１０－６ｍｏｌ／Ｌ、ＮＡＡ０．５４×１０－６～３．２×１０－６ｍｏｌ／Ｌ、ＡｇＮＯ３１．２×１０－５ｍｏｌ／Ｌ的ＭＳ培养基培养子叶切段，能直接－诱导分化出芽和根；３～１１ｄ苗龄的子叶芽分化率稳定在８０％以上，其中４～５ｄ苗龄的效果较好；子叶比下胚轴易产生愈伤组织和再生芽；整片子叶比子叶切段易诱导分化出芽．芽分化率最高可达９７．８％．但每个外植体平均芽数以切去子叶柄的子叶切段为多．激素配比以ＢＡ８．９×１０－６ｍｏｌ／Ｌ、 ＮＡＡ２．７×１０－６ｍｏｌ／Ｌ为最好，在此培养基中再添加８．４×１０－５ｍｏｌ／ＬＣｏ（ＮＯ３）２对促进芽分化有一定作用，但效果不如ＡｇＮＯ３。
In this experiment, the cotyledons of Chinese cabbage “Hybrid Early Hybrid White 02” were used as materials to investigate the various factors affecting the differentiation of shoots during tissue culture. MS medium supplemented with BA4.4 × 10-6 to 35.5 × 10-6 mol / L, NAA0.54 × 10-6 to 3.2 × 10-6 mol / L and AgNO31.2 × 10-5 mol / L Base cotyledons cut directly and induced differentiation of buds and roots. Seedling bud differentiation rate of seedlings aged from 3 to 11 days was stable at more than 80%, of which 4 to 5 days seedling age was better. Cotyledons were easier to produce than hypocotyls Injured tissue and regenerated buds; Cotyledon cotyledons were more likely to induce budding than cotyledons. Bud differentiation rate up to 97.8%. However, the average number of buds per explant was much larger than that of the cotyledon cut into the cotyledons. The ratio of hormones to BA8.9 × 10-6mol / L, NAA2.7 × 10-6mol / L was the best, and then added 8.4 × 10-5mol / LCo (NO3) 2 in this medium to promote the bud Differentiation has a certain effect, but the effect is not as good as AgNO3.