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目的:研究过氧化物酶增殖物激活受体γ(PPARγ)在子宫颈上皮内瘤样变(CIN)与子宫颈癌中的表达及其在子宫颈癌发生、发展中的作用。方法:应用Envision二步免疫组化法进行染色,检测54例CIN和43例子宫颈癌石蜡包埋组织中PPARγ的表达,以20例正常子宫颈组织作为对照进行研究。结果:PPARγ在CINⅠ-Ⅱ中的阳性表达率为44.12%(15/34),在CINⅢ中的阳性表达率为75%(15/20),在子宫颈癌中的阳性表达率为88.37%(38/43),在正常子宫颈组织中的表达率为5.63%。从慢性子宫颈炎、CIN到子宫颈癌,PPARγ的表达呈递增趋势,与正常宫颈组织相比,CIN及子宫颈癌中PPARγ的表达率显著升高(P<0.01)。结论:在CIN及子宫颈癌组织中PPARγ的异常表达提示其与肿瘤的发生、发展密切相关,检测PPARγ有可能成为预测宫颈癌发展及预后的重要指标。
Objective: To investigate the expression of peroxisome proliferator - activated receptor γ (PPARγ) in cervical intraepithelial neoplasia (CIN) and cervical cancer and its role in the development and progression of cervical cancer. Methods: Envision immunohistochemical staining was used to detect the expression of PPARγ in paraffin-embedded tissues of 54 cases of CIN and 43 cases of cervical cancer. Twenty normal cervical tissues were used as control. RESULTS: The positive rate of PPARγ in CINⅠ-Ⅱ was 44.12% (15/34), the positive rate in PPARγ was 75% (15/20) in CINⅢ and the positive rate in cervical cancer was 88.37% ( 38/43), the expression rate in normal cervical tissue was 5.63%. From chronic cervicitis, CIN to cervical cancer, the expression of PPARγ showed an increasing trend. Compared with normal cervix, the expression of PPARγ in CIN and cervical cancer was significantly increased (P <0.01). Conclusion: The abnormal expression of PPARγ in CIN and cervical cancer suggests that it is closely related to the occurrence and development of tumor. Detecting PPARγ may be an important index to predict the development and prognosis of cervical cancer.