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目的：新近发现的新颖细胞周期素依赖激酶抑制剂Ｐ２７在细胞增殖和分化调控中起非常重要作用。本研究目的是探讨流式细胞仪定量Ｐ２７表达在软骨肉瘤中的意义。方法：免疫组化测定软骨肉瘤石蜡切片中Ｐ２７表达，再用流式细胞仪对Ｐ２７表达定量。统计处理用Ｄｕｎｎｅｔｔ－检验和Ｓｐｅａｒｍａｎ秩相关检验。结果：Ｐ２７表达在良、恶性软骨肿瘤间存在极显著差异（ｔ＝５２１，Ｐ＜００１）。Ｐ２７表达与软骨肉瘤分级显著正相关（ｒ＝０９３８，Ｐ＜００５）。软骨肉瘤分级高分化差者，Ｐ２７表达愈高。结论：Ｐ２７蛋白可作为软骨肉瘤诊断标记物和预后指标。
OBJECTIVE: The recently discovered novel cyclin-dependent kinase inhibitor P27 plays an important role in the regulation of cell proliferation and differentiation. The purpose of this study was to investigate the significance of quantitative P27 expression in chondrosarcoma with flow cytometry. Methods: The expression of P27 in paraffin section of chondrosarcoma was determined by immunohistochemistry. The expression of P27 was quantified by flow cytometry. Statistical processing uses Dunnett-test and Spearman rank correlation test. Results: P27 expression was significantly different between benign and malignant cartilage tumors (t=521, P<001). P27 expression was significantly positively correlated with chondrosarcoma grading (r=0938, P<005). Poorly differentiated chondrosarcoma grading, the higher the expression of P27. Conclusion: P27 protein can be used as a diagnostic marker and prognostic indicator of chondrosarcoma.