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[目的]检测溃疡性结肠炎(UC)患者胰岛素样生长因子-1(IGF-1)和基质金属蛋白酶-9(MMP-9)表达变化,并探讨其相关的临床意义。[方法]收集2013年2月~2015年8月我院消化内科诊治的溃疡性结肠炎患者54例,另取同期我院健康体检者40例作为正常对照。ELISA法检测IGF-1和MMP-9表达。[结果]对照组IGF-1表达为(113.64±32.52)ng/ml,显著高于UC组的(68.57±21.38)ng/ml(P<0.05)。对照组MMP-9表达为(16.15±3.23)ng/ml,显著低于UC组的(24.36±5.74)ng/ml(P<0.05)。缓解期患者IGF-1表达为(90.52±28.18)ng/ml,显著高于活动期患者的(45.32±17.52)ng/ml(P<0.05)。缓解期患者MMP-9表达为(18.09±3.07)ng/ml,显著低于活动期患者的(33.42±4.72)ng/ml(P<0.05)。内镜活动性分级Ⅰ、Ⅱ、Ⅲ级溃疡性结肠炎患者之间IGF-1和MMP-9表达存在显著性差异(P<0.05)。其中IGF-1表达Ⅰ级患者高于Ⅱ级患者(P<0.05),Ⅱ级患者又高于Ⅲ级患者(P<0.05);其中MMP-9表达Ⅰ级患者低于Ⅱ级患者(P<0.05),Ⅱ级患者又低于Ⅲ级患者(P<0.05)。[结论]IGF-1和MMP-9异常表达可能在溃疡性结肠炎发病机制中发挥着重要作用,检测二者有可能作为评价溃疡性结肠炎患者病情活动性及严重程度的指标。
[Objective] To investigate the changes of insulin-like growth factor-1 (IGF-1) and matrix metalloproteinase-9 (MMP-9) in patients with ulcerative colitis (UC) [Methods] 54 patients with ulcerative colitis treated in our hospital from February 2013 to August 2015 were enrolled. Another 40 healthy subjects in our hospital were taken as normal control. ELISA was used to detect the expression of IGF-1 and MMP-9. [Results] The expression of IGF-1 in control group was (113.64 ± 32.52) ng / ml, which was significantly higher than that in UC group (68.57 ± 21.38) ng / ml (P <0.05). The expression of MMP-9 in control group was (16.15 ± 3.23) ng / ml, which was significantly lower than that in UC group (24.36 ± 5.74) ng / ml (P <0.05). The expression of IGF-1 in remission patients was (90.52 ± 28.18) ng / ml, which was significantly higher than that in active patients (45.32 ± 17.52) ng / ml (P <0.05). The expression of MMP-9 in remission stage was (18.09 ± 3.07) ng / ml, significantly lower than that in active stage (33.42 ± 4.72) ng / ml (P0.05). There was a significant difference in the expression of IGF-1 and MMP-9 between endoscopic grade I, grade II and grade III ulcerative colitis (P <0.05). The level of IGF-1 was higher in patients with grade I than in patients with grade II (P <0.05), and those in patients with grade II were higher than those in patients with grade III (P <0.05). The level of MMP-9 in patients with grade I was lower than that in patients with grade II (P < 0.05), and those in grade Ⅱ were lower than those in grade Ⅲ (P <0.05). [Conclusion] The abnormal expression of IGF-1 and MMP-9 may play an important role in the pathogenesis of ulcerative colitis. The detection of both may be used as an index to evaluate the activity and severity of ulcerative colitis patients.