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提出了构建一种用于细胞非接触操作的激光微束系统。该系统由 Nd:YAG激光束经声压、热膨胀、汽化等综合效应实现的光刀和 He- Ne激光束经光学动力学效应实现的光镊组成。将两激光束耦合到显微镜中 ,实现了生物细胞的捕获、移动、翻转、打孔等一系列操作。在此基础上 ,分析了形成光镊所必需产生梯度力场的条件和形成光刀对能量的要求 ,进行了系统的总体设计、关键部件设计和选择 ,构建了一套激光微束操作实验系统 ,得到了预期的试验结果。在该系统上成功地实现了非接触细胞操作 ,并对染色体进行了切割。
It is proposed to construct a laser microbeam system for cell non-contact operation. The system is composed of an optical tweezers and an optical tweezers realized by the optical dynamic effect of the optical knife and the He-Ne laser beam which are realized by the comprehensive effects of the sound pressure, the thermal expansion and the vaporization of the Nd: YAG laser beam. The two laser beams coupled to the microscope, to achieve a biological cell capture, move, flip, punch and a series of operations. On this basis, the conditions of forming gradient force field and the requirement of energy for forming optical knife are analyzed. The overall design of the system, The expected test results were obtained. Non-contact cell manipulations were successfully performed on this system and the chromosomes were cut.