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目的探讨外源性P物质与黏着斑激酶、蛋白激酶B、核因子在瘢痕形成中的关联性及时效性。方法采用适宜浓度外源性P物质作用于人胚胎成纤维细胞,应用westenblot蛋白定量观察不同时间段黏着斑激酶、蛋白激酶B、核因子变化情况。结果随着外源性P物质作用时间的延长,人胚胎成纤维细胞中黏着斑激酶、蛋白激酶B、核因子的表达逐渐升高,在30min达到高峰,并至少持续60min。结论外源性P物质可能通过黏着斑激酶、蛋白激酶B、核因子途径迅速起效,有效促进成纤维细胞增殖,从而促进瘢痕形成。
Objective To investigate the association and timeliness of exogenous substance P with focal adhesion kinase, protein kinase B and nuclear factor in the formation of scars. Methods Human embryonic fibroblasts were treated with appropriate concentrations of exogenous substance P, and westenblot protein was used to quantitatively observe the changes of focal adhesion kinase, protein kinase B and nuclear factor at different time points. Results With the prolongation of exogenous substance P, the expression of focal adhesion kinase, protein kinase B and nuclear factor in human embryonic fibroblasts gradually increased and peaked at 30 min and lasted for at least 60 min. Conclusion Exogenous substance P may act rapidly through focal adhesion kinase, protein kinase B and nuclear factor pathway and promote the proliferation of fibroblasts effectively and promote scar formation.