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在Tris缓冲溶液中 ,核酸能与某些碱性三苯甲烷染料如乙基紫 (EthylViolet,EV)、结晶紫 (CrystalViolet,CV)和甲基紫 (MethylViolet,MV)结合而使共振瑞利散射 (RRS)急剧增强并产生新的RRS光谱 .以鲱鱼精DNA (HerringSpermDNA ,hsDNA)为例考察了其相应的光谱特征、影响因素和适宜的反应条件 ,体系均在hsDNA浓度为 0～ 2 0μg/mL时与散射强度呈直线关系 ,方法具有较高的灵敏度 ,其检出限 (3σ)分别为 4 7ng/mL(EV hsDNA体系 ) ,13 0ng/mL (CV hsDNA体系 )和 12 2ng/mL (MV hsDNA体系 ) .考察了某些共存物质的影响 ,并用于合成样品中核酸的测定 ,获得了满意的结果
In Tris buffer solution, the nucleic acid can combine with some basic triphenylmethane dyes such as Ethyl Violet (EV), Crystal Violet (CV) and Methyl Violet (MV) to make Resonance Rayleigh scattering (RRS) was rapidly increased and a new RRS spectrum was generated.The corresponding spectral characteristics, influencing factors and suitable reaction conditions were investigated by using herring sperm DNA (hsDNA) as an example, mL with a linear relationship with the scattering intensity. The detection limit (3σ) was 4 7 ng / mL (EV hsDNA system), 13 0 ng / mL (CV hsDNA system) and 12 2 ng / mL MV hsDNA system.) The effect of some coexisting substances was examined and used for the determination of nucleic acids in synthetic samples with satisfactory results