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通过对5800条大豆疫霉菌EST进行电子查询, 在369条EST中发现415个SSR. 在筛选的EST序列中SSR平均密度为每 8.9 kb含有1个SSR. 在鉴定的SSR中, 三核苷酸重复基元的SSR类型最多, 占鉴定总数的50.1%, 四核苷酸重复基元的SSR类型最少, 为8.2%. 设计40个SSR引物对对5个大豆疫霉菌菌株基因组DNA进行PCR扩增, 有33个引物对扩增出SSR特征条带, 其中有28个引物对扩增出在预期片段大小范围之内的条带. 在33个功能性引物对中, 有15个引物对在5个大豆疫霉菌菌株间扩增出多态性, 多态性引物对占45.5%. 基于SSR标记进行聚类分析, 可将5个检测大豆疫霉菌菌株划分为不同的3个组. 本研究建立了大豆疫霉菌的SSR标记, 为大豆疫霉菌及相关属种的鉴定、遗传变异、分子作图等研究提供了一种更加有效的分子标记系统.
415 SSRs were found in 369 ESTs by electronic query of 5800 Phytophthora sojae ESTs The average SSR density in the screened EST sequences was 1 SSR per 8.9 kb In the identified SSRs the trinucleotide The SSR types of the repeat motifs were the largest, accounting for 50.1% of the total number of identifications, and the tetranucleotide repeat motifs were the least, with 8.2% of the SSR types. Forty SSR primer pairs were designed to amplify genomic DNA of five Phytophthora sojae strains , 33 primer pairs amplified SSR bands, of which 28 pairs amplified the bands within the expected fragment size.In the 33 functional primer pairs, 15 primer pairs in 5 A total of 45 isolates of P. sojae were amplified with polymorphic primers and 45.5% of them were polymorphic. Based on SSR markers, five isolates of Phytophthora sojae were divided into three groups. The SSR markers of Phytophthora sojae provided a more effective molecular marker system for the identification, genetic variation and molecular mapping of Phytophthora sojae and related genera.