论文部分内容阅读
目的研究鼠雾化吸入环丙沙星后血液中环丙沙星的浓度。方法制作气管插管铜绿假单胞菌生物膜感染模型,用生理盐水(第1组),红霉素(第2组),环丙沙星(第3组)和红霉素联合环丙沙星(第4组)雾化干预模型大鼠7天,采用高效液相色谱法测定鼠血浆中环丙沙星的浓度。结果标准曲线回归方程为:y=311.01x-11.333R=0.9999,其线性范围为0.1～10μg/ml。血浆中环丙沙星定量下限为0.1μg/ml。高效液相色谱法测环丙沙星日间和日内精密度RSD均小于10%,体内回收率RSD小于5%,与血浆杂质达到基线分离R>1.5。各样本血浆中环丙沙星浓度均低于环丙沙星标准曲线中所能测定的环丙沙星的最低值0.1ug/ml。结论使用高效液相色谱法测定血浆环丙沙星灵敏、专一、重复性好。雾化方式吸入的环丙沙星几乎不通过局部组织进入血液循环。
Objective To study the concentration of ciprofloxacin in blood after nebulized inhalation of ciprofloxacin in rats. Methods Tracheal intubation model of Pseudomonas aeruginosa biofilm infection was induced by saline (group 1), erythromycin (group 2), ciprofloxacin (group 3) and erythromycin combined with ciprofloxacin Star (group 4) Atomization intervention model rats for 7 days, the concentration of ciprofloxacin in rat plasma was determined by high performance liquid chromatography. Results The standard curve regression equation was: y = 311.01x-11.333R = 0.9999 with a linear range of 0.1-10 μg / ml. The lower limit of quantitation of ciprofloxacin in plasma is 0.1μg / ml. RSD of Ciprofloxacin day and day were less than 10%, RSD of in vivo recovery was less than 5%, and baseline R> 1.5 was separated from plasma impurities. The concentrations of ciprofloxacin in each sample plasma were lower than the lowest value of 0.1 μg / ml for ciprofloxacin in the standard curve of ciprofloxacin. Conclusions Plasma Ciprofloxacin is sensitive, specific and reproducible using HPLC. Inhaled ciprofloxacin atomized into the blood circulation through the local tissue.