【摘 要】
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Dear Editor,The use of sequence-specific nucleases for plant genomic DNA mutagenesis is an exciting and rapidly developing technology (Voytas and Merchant,2013;
【机 构】
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Danziger Innovations Ltd., Mishmar Hashiva Village, Beit Dagan 50297, Israel“,”Precision Biosciences
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Dear Editor,The use of sequence-specific nucleases for plant genomic DNA mutagenesis is an exciting and rapidly developing technology (Voytas and Merchant,2013;Baltes and Voytas,2014).To date,most mutated plants have been recovered from transgenic plants stably expressing nucleases.However,transient nuclease delivery by plant DNA-and RNA-based viral vectors followed by regeneration of plantlets from modified tissues has emerged as an alteative,efficient strategy in some plant species (Marton et al.,2010;Baltes et al.,2014).Delivery of nucleases by plant RNA viruses is particularly attractive because they typically do not integrate into the plant genome,and therefore the mutated plants are not classified as transgenic (Marton et al.,2013;Voytas and Gao,2014).
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