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目的 研究衰老时神经肽Ｙ（ＮＰＹ）阳性神经元在大鼠大脑皮质枕叶中的变化情况。方法 选用青年组（１～２个月）、中年组（１１～１２个月）和老年组（２２～２４个月）三组Ｗｉｓｔａｒ大鼠，采用免疫组化ＳＡＢＣ法结合计算机图像分析技术进行研究。结果 ＮＰＹ阳性细胞属于非锥体形神经元，在枕叶皮质Ⅱ—Ⅵ层均有分布；比较三个年龄组阳性细胞的数量和平均截面积的变化，发现老年组与青年组及中年组相比，两项指标都显著减小（Ｐ＜０．０１），而青年组和中年组相比，两项指标都没有显著性变化（Ｐ＞０．０５）。结论 ＮＰＹ作为神经调节物，可能与大脑皮质枕叶功能的调节有关；随着衰老其阳性神经元的变化可能影响ＮＰＹ发挥正常的生理功能。
Objective To study the changes of neuropeptide Y (NPY) positive neurons in rat cerebral cortex during senescence. Methods Three groups of Wistar rats were selected from the young group (1-2 months), the middle-aged group (11-12 months) and the elderly group (22-24 months). The immunohistochemical SABC method combined with computer image analysis the study. Results The NPY positive cells belonged to non-pyramidal neurons and were distributed in the occipital cortex Ⅱ-Ⅵ. The number of positive cells and the average cross-sectional area of the three age groups were compared. It was found that in the aged group and the young group and in the middle-aged group Compared with the middle-aged group, the two indicators had no significant change (P> 0.05). CONCLUSIONS: NPY, as a neuromodulator, may be involved in the regulation of occipital lobe function in the cerebral cortex. The changes of neurons with aging may affect the normal physiological function of NPY.