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Supernatants collected from the short term in vitro serum-free cultures ofPlasmodium falciparum (Pf) schizont-infected erythrocytes were detected and analysed bycounterimmuno-electrophoresis (CIE) and enzyme-linked immunoabsorbent assay (ELISA).Soluble antigens were purified from the supernatants by immunoabsorbent chroma-tography. It was found that soluble antigens similar to the sonicated Pf antigens inimmunospecificity were present in the supernatants and no erythrocyte membrane antigenswere detectable in the affinity-purified supernatant antigens (Ps-Ag).
Supernatants collected from the short term in vitro serum-free cultures of Plasmodium falciparum (Pf) schizont-infected erythrocytes were detected and analyzed by counterimmuno-electrophoresis (CIE) and enzyme-linked immunoabsorbent assay (ELISA). Soluble antigens were purified from the supernatants by immunoabsorbent chroma-tography. It was found that soluble antigens similar to the sonicated Pf antigens in immunospecificity were present in the supernatants and no erythrocyte membrane antigenswere detectable in the affinity-purified supernatant antigens (Ps-Ag).