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目的探讨癫发作患儿及治疗后血清S-100β、胶质纤维酸性蛋白(GFAP)的变化。方法采用双抗体夹心酶联免疫吸附法对41例癫患儿在癫发作24h内、用药4、12周分别进行定量测定其血清S-100β、GFAP蛋白水平,并与30例健康儿童进行比较。采用SPSS11.0软件进行分析。结果发作后24h:癫组患儿血清S-100β、GFAP蛋白水平均显著高于对照组(Pa<0.01);婴儿痉挛症组血清S-100β、GFAP蛋白水平明显高于其他各组(Pa<0.01);局限性发作与全面性发作组比较,2组S-100β、GFAP蛋白水平无显著性差异(P>0.05)。用药后4周:癫组血清S-100β、GFAP水平较前明显下降,但仍高于健康对照组(P<0.01)。用药后12周:根据癫控制标准,控制23例,显效12例,有效4例,无效2例;各组S-100β、GFAP蛋白水平明显下降,无效组与其他各组比较,S-100β、GFAP蛋白水平仍高(P<0.05);其余各组比较无显著差异。癫患儿血清S-100β、GFAP蛋白水平经直线相关分析呈高度正相关(r=0.54P<0.01)。结论血清S-100β、GFAP水平在发作后癫患儿明显升高,临床控制效果与S-100β、GFAP蛋白水平高低、下降速度有关,二者可作为早期预测脑损伤、损伤程度并判断预后的指标之一。
Objective To investigate the changes of serum S-100β and glial fibrillary acidic protein (GFAP) in children with epileptic seizures. Methods Serum levels of S-100β and GFAP in 41 children with epilepsy were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) at 24h after epileptic seizure, and 30 healthy children Compare Using SPSS11.0 software for analysis. Results Serum levels of S-100β and GFAP in children with epilepsy were significantly higher than those in control group (P <0.01) at 24 h after onset. The levels of S-100β and GFAP in infantile spasms were significantly higher than those in other groups <0.01). There was no significant difference in the levels of S-100β and GFAP in the two groups between limited attack and comprehensive attack (P> 0.05). Four weeks after treatment, the levels of serum S-100β and GFAP in epilepsy group were significantly lower than those in the control group (P <0.01). After 12 weeks of treatment, according to the control standard of epilepsy, 23 cases were controlled, 12 cases were markedly effective, 4 cases were effective and 2 cases were ineffective. The levels of S-100β and GFAP in each group were significantly decreased. , GFAP protein level was still high (P <0.05); the other groups showed no significant difference. Serum levels of S-100β and GFAP in epileptic children were highly correlated with each other by linear correlation analysis (r = 0.54 P <0.01). Conclusions Serum levels of S-100β and GFAP were significantly increased in children with epilepsy after the onset of seizures. The clinical control effect was related to the level of S-100β and GFAP, and the rate of decline. Both of them could be used as early predictors of brain injury, injury and prognosis One of the indicators.